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KMID : 0358419920350081202
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Korean Journal of Obstetrics and Gynecology
1992 Volume.35 No. 8 p.1202 ~ p.1211
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The Effect of Fetal Cord Serum and Protein Supplementation on Two Cell Mouse Embryo Development In Vitro
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임영경/Lim YK
류무현/이여일/Ryu MH/Lee YI
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Abstract
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Two cell stage mouse embryos were cultured in SECM, SECM with 10% human cord serum (HCS), SECM with 4mg/ml bovine serum albumin (BSA), Ham`s F-10, Ham`s F-10 with 10% HCS, Ham`s F-10 sith 4mg/ml BSA, TC 199 with 10% HCS or TC199 with 4mg/ml BSA. Development was assessed by the percent of embryos developed to the morula and blastocyst. Development to the blastocyst stage was significantly higher in Ham`s F-10 with 10% HCS or 4mg/ml BSA than Ham`s F-10 only and also significantly higher in SECM with 10% HCS or 4mg/ml BSA than SECM only. In our results, human cord serum and protein supplement promote the development of two cell stage mouse embryo to the blastocyst stage.
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